Podocalyxin-Like (PODXL) antibody

Details for Product No. ABIN281752
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Synonyms PODXL, podxl, fc18d02, wu:fc18d02, Gp200, PC, PCLP, PCLP-1, AW121214, Ly102, Pclp1, podocalyxin, Gp135, PCLP1, cPCLP1, MEP21, Podxl
(142), (20), (14), (3), (2), (1), (1), (1), (1)
(67), (65), (16), (8), (1)
Clonality (Clone)
Monoclonal ()
(15), (13), (8), (6), (5), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(107), (59), (55), (23), (12), (12), (11), (8), (5), (3), (3), (2), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 100 μg
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Clone TRA-1-60
Isotype IgM
Specificity The TRA160 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA160 antibody is lost.
Alternative Name PODXL (PODXL Antibody Abstract)
Background Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Research Area Cardiovascular, Hematopoietic Progenitors, Extracellular Matrix
Application Notes This TRA -1 -60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 µg per million cells in a 100 µL total staining volume. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200 -250 kDa (reducing and non -reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This TRA -1 -60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.
Restrictions For Research Use only
Buffer Phosphate buffered saline, pH 7.2, less than or equal to 0.09 % NaN3
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C
Supplier Images
anti-Podocalyxin-Like (PODXL) antibody Staining of 2102Ep human embryonal carcinoma cells with 0.25 µg of Purified Mouse IgM...
General Chin, Fong, Goh et al.: "Identification of proteins from feeder conditioned medium that support human embryonic stem cells." in: Journal of biotechnology, Vol. 130, Issue 3, pp. 320-8, 2007 (PubMed).

Schopperle, DeWolf: "The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 3, pp. 723-30, 2007 (PubMed).

Badcock, Pigott, Goepel et al.: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed).

Andrews, Banting, Damjanov et al.: "Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells." in: Hybridoma, Vol. 3, Issue 4, pp. 347-61, 1985 (PubMed).

Validation Images
Catalog No. ABIN281752
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