Podocalyxin-Like (PODXL) antibody

Antigen: Podocalyxin-Like (PODXL)

Synonyms: PC, PCLP, Gp200, MGC138240, Ly102, Pclp1, PCLP-1, AW121214, podocalyxin, PODXL, podxl, fc18d02, wu:fc18d02

Clonality: Monoclonal (TRA-1-60)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN281752

Additional Information

Alternative name: TRA-1-60 (Podocalyxin)

Isotype: IgM

Clone: TRA-1-60

Description: The TRA160 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA160 antibody is lost

Specificity: The TRA -1 -60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA -1 -60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA -1 -60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA -1 -60 recognizes a neurominidase resistent antigen. The TRA -1 -60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34 -related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer., Human

Application Details

Application Notes: This TRA -1 -60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 µg per million cells in a 100 µl total staining volume. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200 -250 kDa (reducing and non -reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This TRA -1 -60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.

Buffer: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3

Storage: Store at 4 °C.Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Images

Staining of 2102Ep human embryonal carcinoma cells with 0.25 µg of Purified Mouse IgM Iso Cntrl (ABIN287102) (open histogram) or 0.25 µg of Purified anti -human TRA -1 -60 (colored histogram) followed by PE anti -mouse IgM (eB121 -15F9) (ABIN286898). Total viable cells were used for analysis.

Publications

Andrews, Banting, Damjanov et al.: "Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells." in: Hybridoma, Vol. 3, Issue 4, pp. 347-61, 1985 (PubMed).

Badcock, Pigott, Goepel et al.: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed).

Schopperle, DeWolf: "The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 3, pp. 723-30, 2007 (PubMed).

Chin, Fong, Goh et al.: "Identification of proteins from feeder conditioned medium that support human embryonic stem cells." in: Journal of biotechnology, Vol. 130, Issue 3, pp. 320-8, 2007 (PubMed).