Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) antibody (APC)
| Antigen |
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| Synonyms |
CD11C, SLEB6, Cr4, N418, Cd11c, AI449405, ITGAX, CD11c |
| Clonality |
Monoclonal (N418) |
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Host
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Reactivity
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Conjugate
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FITC (24), Biotin (18), PE (17), APC (7), PerCP (4), RPE (4), Alexa Fluor 647 (3), Alexa Fluor (2), Alexa Fluor 488 (2), Cy7,PE (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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Flow Cytometry (FACS) (114), Immunofluorescence (IF) (32), Immunoprecipitation (IP) (32), Immunohistochemistry (Fixed) (IHC (fx)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Western Blotting (WB) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (11), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Functional Studies (Func) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN282659 |
| Quantity |
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| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD11c
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Isotype
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IgG
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Clone
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N418
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Specificity
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The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non -covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some, Mouse
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Application Notes
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The N418 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The N418 antibody has been reported for use in flow cytometric analysis.
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Buffer
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Phosphate buffer pH 7.2,150 mM NaCl, 0.09% NaN3
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Storage
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Store at 4 °C.DO NOT FREEZE.LIGHT -SENSITIVE MATERIAL.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Kaplan, Li, Jenison et al.: "Autocrine/paracrine TGFbeta1 is required for the development of epidermal Langerhans cells." in: The Journal of experimental medicine, Vol. 204, Issue 11, pp. 2545-52, 2007 (PubMed).
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Alternatives for antigen "Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX)", type "Antibodies"
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Hosts
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Mouse (97), Rabbit (36), Hamster (19), Armenian Hamster (4), Chicken (1), Rat (1)
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Reactivities
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Human (125), Mouse (Murine) (46), Dog (Canine) (30), Rat (Rattus) (25), Monkey (7), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1)
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Applications
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Flow Cytometry (FACS) (114), Immunofluorescence (IF) (32), Immunoprecipitation (IP) (32), Immunohistochemistry (Fixed) (IHC (fx)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Western Blotting (WB) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (11), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Functional Studies (Func) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (24), Biotin (18), PE (17), APC (7), PerCP (4), RPE (4), Alexa Fluor 647 (3), Alexa Fluor (2), Alexa Fluor 488 (2), Cy7,PE (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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Internal Region (3), C-Term (2), AA 287-339 (1)
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Alternatives 
2013 BioMed Central Research Awards
