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TPTEa antibody (N-Term)

Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB4973 unconjugated
Catalog No. ABIN2841038
  • Target See all TPTEa products
    TPTEa
    Binding Specificity
    • 7
    • 6
    • 1
    AA 42-74, N-Term
    Reactivity
    Human
    Host
    • 7
    Rabbit
    Clonality
    • 7
    Polyclonal
    Conjugate
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This TPTEa antibody is un-conjugated
    Application
    • 7
    • 6
    • 6
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    This antibody is purified through a protein A column, followed by peptide affinity purification.
    Immunogen
    This TPTEa antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-74 amino acids from the N-terminal region of human TPTEa.
    Clone
    RB4973
    Isotype
    Ig Fraction
  • Application Notes
    WB: 1:1000. IHC-P: 1:10~50
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    Expiry Date
    6 months
  • Target
    TPTEa
    Abstract
    TPTEa Products
    Background
    Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
    Gene ID
    7179
    NCBI Accession
    NP_954868, NP_954869, NP_954870
    UniProt
    P56180
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