ALDH1A3 antibody (N-Term)

Catalog No. ABIN2841966

Product Details anti-ALDH1A3 Antibody

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Binding Specificity: AA 24-51, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ALDH1A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ALDH1A3.

Clone: RB16818

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-ALDH1A3 antibody (ABIN2841966)

Petrachi, Romagnani, Albini, Longo, Argenziano, Grisendi, Dominici, Ciarrocchi, Dallaglio: "Therapeutic potential of the metabolic modulator phenformin in targeting the stem cell compartment in melanoma." in: Oncotarget, Vol. 8, Issue 4, pp. 6914-6928, (2018) (PubMed).

Pérez-Alea, McGrail, Sánchez-Redondo, Ferrer, Fournet, Cortés, Muñoz, Hernandez-Losa, Tenbaum, Martin, Costello, Ceylan, Garcia-Patos, Recio: "ALDH1A3 is epigenetically regulated during melanocyte transformation and is a target for melanoma treatment." in: Oncogene, Vol. 36, Issue 41, pp. 5695-5708, (2017) (PubMed).

Kent, Arnold, Fasnacht, Rowsey, Mitchell, Hogarth, Isoherranen, Griswold: "ALDH Enzyme Expression Is Independent of the Spermatogenic Cycle, and Their Inhibition Causes Misregulation of Murine Spermatogenic Processes." in: Biology of reproduction, Vol. 94, Issue 1, pp. 12, (2016) (PubMed).

Martinez-Cruzado, Tornin, Santos, Rodriguez, García-Castro, Morís, Rodriguez: "Aldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations." in: Scientific reports, Vol. 6, pp. 27878, (2016) (PubMed).

Kashii-Magaribuchi, Takeuchi, Haisa, Sakamoto, Itoh, Izawa, Isa, Fukuzawa, Murakami, Takahashi: "Induced Expression of Cancer Stem Cell Markers ALDH1A3 and Sox-2 in Hierarchical Reconstitution of Apoptosis-resistant Human Breast Cancer Cells." in: Acta histochemica et cytochemica, Vol. 49, Issue 5, pp. 149-158, (2016) (PubMed).

Golubovskaya, OBrien, Ho, Heffler, Conroy, Hu, Wang, Liu, Cance: "Down-regulation of ALDH1A3, CD44 or MDR1 sensitizes resistant cancer cells to FAK autophosphorylation inhibitor Y15." in: Journal of cancer research and clinical oncology, Vol. 141, Issue 9, pp. 1613-31, (2015) (PubMed).

Arnold, Kent, Hogarth, Schlatt, Prasad, Haenisch, Walsh, Muller, Griswold, Amory, Isoherranen: "Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations." in: Journal of lipid research, Vol. 56, Issue 2, pp. 342-57, (2015) (PubMed).

Shao, Sullivan, Girard, Augustyn, Yenerall, Rodriguez-Canales, Liu, Behrens, Shay, Wistuba, Minna: "Essential role of aldehyde dehydrogenase 1A3 for the maintenance of non-small cell lung cancer stem cells is associated with the STAT3 pathway." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 15, pp. 4154-66, (2014) (PubMed).

Selvam, Wong, Flanagan, Prasad: "Cellular level classification of breast cancer through proteomic markers using nanochannel array sensors." in: Nanomedicine (London, England), (2013) (PubMed).

Target Details for ALDH1A3

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Alternative Name: ALDH1A3 (ALDH1A3 Products)

Synonyms: RALDH3 antibody, ALDH1A3 antibody, ALDH1A6 antibody, ALDH6 antibody, MCOP8 antibody, Aldh6 antibody, V1 antibody, aldehyde dehydrogenase 1 family member A3 antibody, aldehyde dehydrogenase 1 family member A3 L homeolog antibody, aldehyde dehydrogenase 1 family, member A3 antibody, aldehyde dehydrogenase family 1, subfamily A3 antibody, ALDH1A3 antibody, aldh1a3.L antibody, aldh1a3 antibody, Aldh1a3 antibody

Background: Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme ALDH1A3 uses retinal as a substrate, either in a free or cellular retinol-binding protein form.

Molecular Weight: 56108

Gene ID: 220

NCBI Accession: NP_000684

UniProt: P47895

Pathways: Retinoic Acid Receptor Signaling Pathway