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DYKDDDDK Tag antibody

Reactivity: Human WB Host: Rabbit Polyclonal RB0493 unconjugated
Catalog No. ABIN2843193
  • Target See all DYKDDDDK Tag products
    DYKDDDDK Tag
    Reactivity
    • 4
    • 2
    • 2
    • 2
    • 1
    Human
    Host
    • 58
    • 52
    • 8
    • 6
    • 6
    Rabbit
    Clonality
    • 65
    • 64
    Polyclonal
    Conjugate
    • 46
    • 15
    • 10
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DYKDDDDK Tag antibody is un-conjugated
    Application
    • 80
    • 62
    • 29
    • 24
    • 19
    • 17
    • 12
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    DYKDDDDK
    Purification
    This antibody is purified through a protein A column, followed by peptide affinity purification.
    Immunogen
    KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
    Clone
    RB0493
    Isotype
    IgG
  • Application Notes
    WB: 1:2000. WB: 1:2000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    Expiry Date
    6 months
  • Xiao, Gao, Hou, Xu, Chang, Wang, Hu, He, Luo, Wang, Peng, Tang, Zhu, Xiong: "Chromatin-remodelling factor Brg1 regulates myocardial proliferation and regeneration in zebrafish." in: Nature communications, Vol. 7, pp. 13787, (2018) (PubMed).

    Al-Sady, Greenstein, El-Samad, Braun, Madhani: "Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics." in: PLoS ONE, Vol. 11, Issue 8, pp. e0159292, (2017) (PubMed).

  • Target
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag Products
    Target Type
    Tag
    Background
    Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).
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