For best experience we recommend to activate Javascript in your browser.

His Tag antibody

Name: His Tag antibody
SKU: ABIN2843197
Price: 181.5 USD
Availability: InStock

WB Host: Rabbit Polyclonal RB19955 unconjugated

Western blot analysis of His Tag Antibody (ABIN387901 and ABIN2843197) in his-tag TBB1 protein (35 μg/lane).

All lanes : Anti-His Tag Antibody at 1:2000 dilution Lane 1: 12-tag recombinant protein lysate Lysates/proteins at 20 μg per lane.

All lanes : Anti-His Tag Antibody at 1:2000 dilution Lane 1: recombinant protein with His tag lysate Lysates/proteins at 20 μg per lane.

3 images

(1 reference)

Catalog No. ABIN2843197

Datasheet as PDF

Target See all His Tag Antibodies

His Tag

Reactivity

Please inquire
Host
93

56

12

9

8

7

2

1
Rabbit
Compatible Secondaries
Clonality
106

69

10
Polyclonal
Conjugate
91

22

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
Application
153

103

38

27

25

17

14

14

14

13

13

7

7

6

6

5

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB)

Sequence

HHHHHH

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

Poly-HIS peptide were used to produced this antibody.

Clone

RB19955

Isotype

IgG

Top Product

Discover our top product His Tag Primary Antibody
anti-His Tag antibody
WB, ELISA, IP, IHC Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
Reactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS Host: Mouse Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
Reactivity: Tag, Human WB Host: Mouse Monoclonal 2D5 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 HRP

anti-His Tag antibody
Reactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated

anti-His Tag (C-Term) antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 Biotin

Application Notes

WB: 1:2000. WB: 1:2000. WB: 1:1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Anderson, Robson, Jiang, Malmirchegini, Fierobe, Lazazzera, Clubb: "Assembly of minicellulosomes on the surface of Bacillus subtilis." in: Applied and environmental microbiology, Vol. 77, Issue 14, pp. 4849-58, (2011) (PubMed).
Target

His Tag

Alternative Name

6 X His Tag (His Tag Products)

Target Type

Tag

Background

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.