Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
Specificity
This antibody is specific for bromodeoxyuridine and does not cross react with thymidine.
Cross-Reactivity
Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus)
Cross-Reactivity (Details)
This antibody reacts with the 85 kDa ß-chain of the human LRP/alpha MR which 2is expressed in a restricted spectrum of cell types. Cross-reacts with rabbit LDL receptor-related protein (LRP) or mouse LRPAntigen detected in Human, rabbit, rat, hamster and mouse.
Immunogen
LRP antibody (85 kDa) was raised in mouse using human LRP/a2 MR as the immunogen.
IHC: 1:20 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in dist. water (final solution contains 0.5 % BSA in PBS buffer, pH 7.4)
Concentration
Lot specific
Buffer
Lyophilized.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Bornachea, Benitez-Amaro, Vea, Nasarre, de Gonzalo-Calvo, Escola-Gil, Cedo, Iborra, Martínez-Martínez, Juarez, Camara, Espinet, Borrell-Pages, Badimon, Castell, Llorente-Cortés: "Immunization with the Gly1127-Cys1140 amino acid sequence of the LRP1 receptor reduces atherosclerosis in rabbits. Molecular, immunohistochemical and nuclear imaging studies." in: Theranostics, Vol. 10, Issue 7, pp. 3263-3280, (2020) (PubMed).
de Gonzalo-Calvo, López-Vilaró, Nasarre, Perez-Olabarria, Vázquez, Escuin, Badimon, Barnadas, Lerma, Llorente-Cortés: "Intratumor cholesteryl ester accumulation is associated with human breast cancer proliferation and aggressive potential: a molecular and clinicopathological study." in: BMC cancer, Vol. 15, pp. 460, (2016) (PubMed).