APP antibody was raised in goat using a synthetic peptide corresponding to amino acids 44-63 of the N-terminus of the alzheimer's disease APP as the immunogen.
ELISA: 1:5,000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Concentration
Lot specific
Buffer
PBS, pH 7.4 with 0.05 % NaN3.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Gasparini, Crowther, Martin, Berg, Coleman, Goedert, Spillantini: "Tau inclusions in retinal ganglion cells of human P301S tau transgenic mice: effects on axonal viability." in: Neurobiology of aging, Vol. 32, Issue 3, pp. 419-33, (2011) (PubMed).
Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (ABeta), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients.