IL3RA antibody (FITC)

Catalog No. ABIN2853580

Product Details anti-IL3RA Antibody (FITC)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with Human CD123 antigen (IL-3 receptor alpha-chain).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein-A Sepharose Chromatography.

Immunogen: COS cells overexpressing IL-3 receptor alpha-chain. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte

Clone: 6H6

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Transfectant cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric Aanalysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 μL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD123 monoclonal antibody (10-20 μg/mL) diluted with thewashing buffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum and 0. 1% NaN3(5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD123 monoclonal antibody into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 μL of the non diluted FITC labeled CD123 monoclonal antibody into each tube. 2) Add 50 μL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulterinstruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Resuspend the cells with 500 μL of the washing buffer [PBS containing 2% fetal calfserum (FCS) and 0. 1% NaN3] and analyze by a flow cytometer. Positive Control for Flow Cytometry: Peripheral blood monocytes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.05 mg/mL

Buffer: PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123 / IL3RA (IL3RA Products)

Synonyms: CD123 antibody, IL3R antibody, IL3RAY antibody, IL3RX antibody, IL3RY antibody, hIL-3Ra antibody, CDw123 antibody, SUT-1 antibody, Cyrl antibody, interleukin 3 receptor subunit alpha antibody, interleukin 3 receptor, alpha chain antibody, IL3RA antibody, Il3ra antibody

Background: CD123 is the IL-3-specific component of the IL-3 receptor. CD123 binds IL-3 only in combination with CDw131, the Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha

Gene ID: 3563

NCBI Accession: NP_002174

UniProt: P26951

Pathways: JAK-STAT Signaling