Sca-1/Ly-6A/E antibody (Biotin)

Catalog No. ABIN2853588

Product Details anti-Sca-1/Ly-6A/E Antibody (Biotin)

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts withSca-1.

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Protein-G agarose

Immunogen: Mouse Sca-1 transfected LO cells

Clone: 238B

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % NaN3, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly6A/E / SCA1 (Ly6a Products)

Background: Sca-1 is a member of the Ly-6 antigen family which molecular mass of 8 kDa under non-reducing conditions and of 18 kDa under reducing conditions. Mouse hematopoietic stem cells express low levels of Thy-1 antigen (Thy-1lo) and to be negative for lineage markers (Lin-) that is, not expressing markers characteristic of B cells (B220), granulocytes (Gr-1), myelomonocytic cells (Mac-1) and T lymphocytes (CD4 and CD8). Recently, new monoclonal antibody, anti-Sca-1, was used to purify stem cells from the Thy-1lo, Linbone marrow subpopulation. Thy-1lo, Lin-, Sca-1+ (but not the Thy-1lo, Lin-, Sca-1-) population of bone marrow cells are highly purified pluripotent stem cells. They read out with nearly unit efficiency in assays for primitive myeloerythroid and thymic progenitor, and have a capability to admit lethally irradiated mouse to survive and be restored in all blood-cell lineages. The Thy-1lo, Lin-, Sca-1+ subpopulation thought to have all stem cells present in the bone marrow.Synonyms: Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP

Gene ID: 110454

NCBI Accession: NP_034868

UniProt: P05533

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation