CXCR4 antibody (Extracellular Domain, N-Term) (FITC)

Catalog No. ABIN2853593

Product Details anti-CXCR4 Antibody (FITC)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Binding Specificity: Extracellular Domain, N-Term

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CXCR4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This clone A145 recognizes N-terminus extracellular domain of CXCR4.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulfate precipitation followed by gel filtration through Superdex 200 in PBS

Immunogen: CXCR4 transfected Cos-1 cells

Clone: A145

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, 0.09 % NaN3, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CD184 / CXCR4 (CXCR4 Products)

Background: CXCR4/CD184/LESTR/fusin/NPY3R is a G protein-coupled receptor for the CXC chemokine SDF-1. Binding of SDF-1 induces CXCR4 phosphorylation by Ser/Thr kinases, leading to CXCR4 internalization via clathrin-coated pits. CXCR4 functions include co-stimulation in pre-B cell proliferation, induction of apoptosis, and HIV entry, since CXCR4 is one of the 2 major HIV/SIV co-receptors. Early infection with HIV-1 is dominated by CCR5-tropic (R5) viruses. The evolution of CXCR4-tropic (X4) viruses occurs later in the infection and is associated with rapid disease progression. CXCR4 mediates chemotaxis in mature and progenitor blood cells and is essential for B lympho- and myelopoiesis, cardiogenesis, blood vessel formation and cerebellar development. Although ubiquitously expressed in blood and tissue cells, its role in blood and tissue homeostasis is not fully understood. CXCR4 is predominantly stored intracellularly, and may contribute to the inefficiency in transmission and propagation of X4-tropic viruses.Synonyms: C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, NPYRL, SDF1 receptor, Stromal cell-derived factor 1 receptor

Gene ID: 7852

NCBI Accession: NP_001008540

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events