Myc Tag antibody (Agarose Beads)

Details for Product No. ABIN2853622, Supplier: Log in to see
Antigen
Reactivity
Tag
214
36
14
10
6
5
3
1
1
Host
Mouse
160
62
17
16
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Agarose Beads
27
23
14
8
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP)
218
116
79
53
48
35
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen GST-6myc-Tag fusion protein
Sequence EQKLISEEDL
Clone PL14
Isotype IgG1
Specificity This antibody recognizes Myc-Tag peptide sequence (EQKLISEEDL).
Purification Protein A agarose
Alternative Name C-Myc Epitope Tag
Target Type Tag
Background Epitope tagging is a widely accepted technique that fuses an epitope-containing peptide to a target protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VAV-G- Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
Gene ID 4609
NCBI Accession NP_002458
UniProt P01106
Research Area Transcription Factors, Cancer, Cell Cycle
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 200 μg of anti-Myc Tag monoclonal antibody covalently coupled to 200 μL of agarose gel and provided as a 50 % gel slurry suspended in PBS containing preservative (0.09 % sodium azide) for a total volume of 400 μL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Supplier Images
Western Blotting (WB) image for anti-Myc Tag antibody (Agarose Beads) (ABIN2853622) anti-Myc Tag antibody (Agarose Beads)
Background publications Hirota, Daitoku, Matsuzaki, Araya, Yamagata, Asada, Sugaya, Fukamizu: "Hepatocyte nuclear factor-4 is a novel downstream target of insulin via FKHR as a signal-regulated transcriptional inhibitor." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13056-60, 2003 (PubMed).

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002 (PubMed).

Bae, Ahn, Jeong, Bae, Lee, Bae, Park, Kim, Kim: "Jab1 interacts directly with HIF-1alpha and regulates its stability." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 9-12, 2002 (PubMed).

Tojo, Matsuzaki, Minami, Honda, Yasuda, Chiba, Saya, Fujii-Kuriyama, Nakao: "The aryl hydrocarbon receptor nuclear transporter is modulated by the SUMO-1 conjugation system." in: The Journal of biological chemistry, Vol. 277, Issue 48, pp. 46576-85, 2002 (PubMed).

"In Celebration of 100 Years of Publication." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 1, 2001 (PubMed).

Mukai, Hachiya, Shoji-Hoshino, Kimura, Nadano, Suvanto, Hanaoka, Li, Irie, Greene, Sato: "NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR." in: The Journal of biological chemistry, Vol. 275, Issue 23, pp. 17566-70, 2000 (PubMed).

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