Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Cross-Reactivity
Human, Mouse, Rat
Characteristics
Rabbit Polyclonal antibody to Endothelin B Receptor (endothelin receptor type B) Endothelin B Receptor antibody
Purification
Purified by antigen-affinity chromatography.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of rat Endothelin B Receptor. The exact sequence is proprietary.
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
1XPBS ( pH 7), 50 % Glycerol, 0.01 % Thimerosal
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.