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SQSTM1 antibody

KO Validated SQSTM1 Reactivity: Human WB, IF, IP, IHC (p), ICC, FACS, PLA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2854836
  • Target See all SQSTM1 Antibodies
    SQSTM1 (Sequestosome 1 (SQSTM1))
    Reactivity
    • 171
    • 64
    • 61
    • 18
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 145
    • 24
    • 1
    • 1
    Rabbit
    Clonality
    • 144
    • 27
    Polyclonal
    Conjugate
    • 77
    • 13
    • 12
    • 8
    • 8
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This SQSTM1 antibody is un-conjugated
    Application
    • 121
    • 58
    • 49
    • 40
    • 24
    • 22
    • 17
    • 16
    • 14
    • 13
    • 9
    • 5
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Proximity Ligation Assay (PLA)
    Cross-Reactivity
    Bee, Cow, Human, Mosquito, Mouse, Rat, Zebrafish (Danio rerio)
    Characteristics
    Rabbit Polyclonal antibody to SQSTM1 (sequestosome 1)
    SQSTM1 antibody [N3C1], Internal
    Purification
    Purified by antigen-affinity chromatography.
    Grade
    KO Validated
    Immunogen
    Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.
    Isotype
    IgG
    Top Product
    Discover our top product SQSTM1 Primary Antibody
  • Application Notes
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Comment

    Positive Control: NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 ( 3μM Thapsigargin 12hr) , HepG2

    Validation: Comparison, KO/KD, Orthogonal

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.39 mg/mL
    Buffer
    1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Liu, Jiang, Wu, Wu, Wu, Zhao, Sun, Huang, Li, Sheng, Barbier, Cintrat, Gillet, Su: "Antiviral Effects of ABMA and DABMA against Influenza Virus In Vitro and In Vivo via Regulating the Endolysosomal Pathway and Autophagy." in: , Vol. 23, Issue 7, (2022) (PubMed).

    Cheung, Hsu, Lin, Chen, Wang, Huang, Juan: "ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 75, (2020) (PubMed).

    Chou, Lin, Dzhagalov, Chen, Lin, Lin, Chen, Chen, Fu: "Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy." in: Scientific reports, Vol. 10, Issue 1, pp. 8422, (2020) (PubMed).

    Jensen, Schuldi, McAvoy, Russell, Boehringer, Curran, Krishnamurthy, Wen, Westergard, Ma, Haeusler, Edbauer, Pasinelli, Trotti: "Synaptic dysfunction induced by glycine-alanine dipeptides in C9orf72-ALS/FTD is rescued by SV2 replenishment." in: EMBO molecular medicine, Vol. 12, Issue 5, pp. e10722, (2020) (PubMed).

    Martello, Lauriola, Mellis, Parish, Dawson, Imrie, Vidmar, Gammoh, Mitić, Brittan, Mills, Carragher, DArca, Caporali: "Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy." in: EMBO reports, Vol. 21, Issue 7, pp. e48192, (2020) (PubMed).

    Wong, Chiu, Tu, Sheu, Chan: "Anticancer Effects of Antihypertensive L-Type Calcium Channel Blockers on Chemoresistant Lung Cancer Cells via Autophagy and Apoptosis." in: Cancer management and research, Vol. 12, pp. 1913-1927, (2020) (PubMed).

    Hsieh, Li, Hsu, Chen, Chen, Liu, Liu, Kuo, Liu: "Mitochondrial protection by simvastatin against angiotensin II-mediated heart failure." in: British journal of pharmacology, Vol. 176, Issue 19, pp. 3791-3804, (2020) (PubMed).

    Nombela, Requena-Platek, Morales-Lange, Chico, Puente-Marin, Ciordia, Mena, Coll, Perez, Mercado, Ortega-Villaizan: "Rainbow Trout Red Blood Cells Exposed to Viral Hemorrhagic Septicemia Virus Up-Regulate Antigen-Processing Mechanisms and MHC I&II, CD86, and CD83 Antigen-presenting Cell Markers." in: Cells, Vol. 8, Issue 5, (2019) (PubMed).

    Lin, Lin, Chen, Yang, Hung: "Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 1, (2019) (PubMed).

    Chiu, Hsu, Hsu, Huang, Liu, Wu, Huang, Chen, Chen, Chiu: "Human non‑small cell lung cancer cells can be sensitized to camptothecin by modulating autophagy." in: International journal of oncology, Vol. 53, Issue 5, pp. 1967-1979, (2019) (PubMed).

    Yi, Wang, Du, He, Ho, Qiu, Zou, Li, Jin, Zhan, Zhao, Liu: "Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells." in: Molecules (Basel, Switzerland), Vol. 23, Issue 6, (2018) (PubMed).

    Zheng, Li, Li, Zhu, Cui, Wong, Chang, Liao: "Induction of N-Ras degradation by flunarizine-mediated autophagy." in: Scientific reports, Vol. 8, Issue 1, pp. 16932, (2018) (PubMed).

    Chiu, Hu, Yang, Hsin, Ko, Tsai, Sheu: "Immunomodulatory Protein from Ganoderma microsporum Induces Pro-Death Autophagy through Akt-mTOR-p70S6K Pathway Inhibition in Multidrug Resistant Lung Cancer Cells." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125774, (2015) (PubMed).

  • Target
    SQSTM1 (Sequestosome 1 (SQSTM1))
    Alternative Name
    sequestosome 1 (SQSTM1 Products)
    Synonyms
    SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody
    Background
    This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

    Cellular Localization: Cytoplasm , Late endosome , Nucleus
    Molecular Weight
    48 kDa
    Gene ID
    8878
    UniProt
    Q13501
    Pathways
    NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
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