FLNB antibody (N-Term)

Catalog No. ABIN2854971

Product Details anti-FLNB Antibody

Target: FLNB (Filamin B, beta (FLNB))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLNB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Filamin B (filamin B, beta)
Filamin B antibody [N1], N-term

Purification: Affinity purified by Protein A.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , NIH-3T3 , 293T , A431 , HeLa , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-FLNB antibody (ABIN2854971)

Artym, Swatkoski, Matsumoto, Campbell, Petrie, Dimitriadis, Li, Mueller, Bugge, Gucek, Yamada: "Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network." in: The Journal of cell biology, Vol. 208, Issue 3, pp. 331-50, (2015) (PubMed).

Target Details for FLNB

Target: FLNB (Filamin B, beta (FLNB))

Alternative Name: filamin B (FLNB Products)

Synonyms: ABP-278 antibody, ABP-280 antibody, AOI antibody, FH1 antibody, FLN-B antibody, FLN1L antibody, LRS1 antibody, SCT antibody, TABP antibody, TAP antibody, AL024016 antibody, filamin B antibody, filamin, beta antibody, FLNB antibody, Flnb antibody

Background: This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3, boomerang dysplasia, autosomal dominant Larsen syndrome, and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Cellular Localization: Isoform 1: Cytoplasm , cell cortex , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Cytoplasm

Molecular Weight: 278 kDa

Gene ID: 2317

UniProt: O75369

Pathways: Maintenance of Protein Location