PHGDH antibody (N-Term)
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- Target See all PHGDH Antibodies
- PHGDH (phosphoglycerate Dehydrogenase (PHGDH))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PHGDH antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Cross-Reactivity
- Human, Mouse, Rat
- Characteristics
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Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)
PHGDH antibody [N1N2], N-term - Purification
- Purified by antigen-affinity chromatography.
- Grade
- KO Validated
- Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product PHGDH Primary Antibody
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anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibody
PHGDH Reactivity: Human WB, ELISA, IHC (p), IF, IP Host: Mouse Monoclonal 4A3-1D6 unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 264-533) antibodyPHGDH Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 140-168) antibodyPHGDH Reactivity: Human, Mouse WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 2-251) antibodyPHGDH Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 140-168), (N-Term) antibodyPHGDH Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB20882 unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibodyPHGDH Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB20883 unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibodyPHGDH Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (N-Term) antibodyPHGDH Reactivity: Human, Mouse, Rat WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibodyPHGDH Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibodyPHGDH Reactivity: Human WB Host: Mouse Polyclonal unconjugated
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: Mouse brain , 293T , A431 , HeLaS3 , HeLa
Validation: KO/KD
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.87 mg/mL
- Buffer
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- PHGDH (phosphoglycerate Dehydrogenase (PHGDH))
- Alternative Name
- phosphoglycerate dehydrogenase (PHGDH Products)
- Background
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3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]
- Molecular Weight
- 57 kDa
- Gene ID
- 26227
- UniProt
- O43175
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Warburg Effect
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