MAP3K8 antibody

Catalog No. ABIN2855464

Product Details anti-MAP3K8 Antibody

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to MAP3K8 (mitogen-activated protein kinase kinase kinase 8)
MAP3K8 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MAP3K8. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Lovo

Validation: Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.33 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MAP3K8

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Alternative Name: mitogen-activated protein kinase kinase kinase 8 (MAP3K8 Products)

Synonyms: MAP3K8 antibody, COT antibody, EST antibody, ESTF antibody, MEKK8 antibody, TPL2 antibody, Tpl-2 antibody, c-COT antibody, Cot antibody, Cot/Tpl2 antibody, Est antibody, Estf antibody, Tpl2 antibody, D17TPL2 antibody, mitogen-activated protein kinase kinase kinase 8 antibody, MAP3K8 antibody, map3k8 antibody, Map3k8 antibody

Background: This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.

Molecular Weight: 53 kDa

Gene ID: 1326

UniProt: P41279

Pathways: PI3K-Akt Signaling, TCR Signaling