PYGB antibody (C-Term)

Catalog No. ABIN2855804

Product Details anti-PYGB Antibody

Target: PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYGB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: Rabbit Polyclonal antibody to GPBB (phosphorylase, glycogen, brain)
GPBB antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GPBB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HepG2 , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.84 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PYGB antibody (ABIN2855804)

Cooper, Pasini, Lambert, DAlessandro, Yao, Risner, Calkins: "Redistribution of metabolic resources through astrocyte networks mitigates neurodegenerative stress." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 117, Issue 31, pp. 18810-18821, (2020) (PubMed).

Geiszler, Ugun-Klusek, Lawler, Pardon, Yuchun, Bai, Daykin, Auer, Bedford: "Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons." in: Scientific reports, Vol. 8, Issue 1, pp. 4833, (2019) (PubMed).

Target Details for PYGB

Target: PYGB (GPBB) (phosphorylase, Glycogen, Brain (GPBB))

Alternative Name: GPBB (GPBB Products)

Synonyms: GPBB antibody, GLYPHOA antibody, glycogen phosphorylase B antibody, phosphorylase, glycogen; brain antibody, brain glycogen phosphorylase antibody, phosphorylase, glycogen; brain S homeolog antibody, PYGB antibody, pygb antibody, Pygb antibody, pygb.S antibody

Background: The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.

Molecular Weight: 97 kDa

Gene ID: 5834

Pathways: Cellular Glucan Metabolic Process