PRKACA antibody (Protein Kinase A, alpha) (Center)

Details for Product anti-PRKACA Antibody No. ABIN2855997, Supplier: Log in to see
Antigen
  • PKCD
  • Pkaca
  • PKACA
  • PKA
  • Calpha
  • Cs-PKA
  • PKCA1
  • PRKAR1
  • protein kinase, cAMP dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, regulatory, type I, beta
  • Prkaca
  • PRKACA
  • PRKAR1B
Alternatives
anti-Cow (Bovine) PRKACA antibody for Immunoprecipitation
Epitope
Center
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16
12
7
7
4
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3
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2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
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29
24
4
3
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2
2
2
2
2
Host
Rabbit
62
43
6
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
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4
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2
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2
1
1
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1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
95
42
15
13
11
10
5
4
3
2
2
1
1
Options
Supplier
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Immunogen Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.
Isotype IgG
Cross-Reactivity Rat (Rattus), Dog (Canine), Sheep (Ovine), Cow (Bovine)
Cross-Reactivity (Details) Rat (100 %), Dog (100 %), Sheep (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to PKA C alpha (protein kinase, cAMP-dependent, catalytic, alpha)
PKA C alpha antibody [N2C3]
Purification Purified by antigen-affinity chromatography.
Alternative Name PKA C alpha (PRKACA Antibody Abstract)
Background CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Cytoplasm (By similarity) , Nucleus (By similarity)
Molecular Weight 41 kDa
Gene ID 5566
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HeLa , Jurkat , NT2D1 , mouse brain

Restrictions For Research Use only
Format Liquid
Concentration 0.38 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) WB Image Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE antibody diluted at...
Western Blotting (WB) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon, using PKA ...
Immunofluorescence (IF) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PKA alpha, ant...
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