NSFL1C antibody (Center)

Catalog No. ABIN2856019

Product Details anti-NSFL1C Antibody

Target: NSFL1C (NSFL1 (p97) Cofactor (p47) (NSFL1C))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSFL1C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Cross-Reactivity: Rhesus Monkey

Cross-Reactivity (Details): Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to NSFL1C (NSFL1 (p97) cofactor (p47))
NSFL1C antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NSFL1C. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NSFL1C

Target: NSFL1C (NSFL1 (p97) Cofactor (p47) (NSFL1C))

Alternative Name: NSFL1C (NSFL1C Products)

Synonyms: zehn2383 antibody, wu:fc88f08 antibody, zgc:101636 antibody, hm:zehn2383 antibody, P47 antibody, UBX1 antibody, UBXD10 antibody, UBXN2C antibody, dJ776F14.1 antibody, Munc-18c antibody, Stxbp3a antibody, p47 antibody, XY40 antibody, NSFL1 cofactor antibody, NSFL1 (p97) cofactor (p47) antibody, NSFL1C antibody, nsfl1c antibody, Nsfl1c antibody

Background: N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene.

Cellular Localization: Nucleus (By similarity) , Golgi apparatus , Golgi stack (By similarity)

Molecular Weight: 41 kDa

Gene ID: 55968