GLO1 antibody

Catalog No. ABIN2856190

Product Details anti-GLO1 Antibody

Target: GLO1 (Glyoxalase I (GLO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Glyoxalase I (glyoxalase I)
Glyoxalase I antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-GLO1 antibody (ABIN2856190)

Chen, Lin, Chen, Cheng, Cheng, Imai et al.: "Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." in: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).

Target Details for GLO1

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: glyoxalase I (GLO1 Products)

Synonyms: GLOD1 antibody, GLYI antibody, 0610009E22Rik antibody, 1110008E19Rik antibody, 2510049H23Rik antibody, AW550643 antibody, GLY1 antibody, Glo-1 antibody, Glo-1r antibody, Glo-1s antibody, Glo1-r antibody, Glo1-s antibody, Qglo antibody, cb554 antibody, zgc:66035 antibody, wu:fb82g09 antibody, glod1 antibody, glyi antibody, glo1 antibody, GLO1 antibody, ATGLX1 antibody, F12F1.32 antibody, F12F1_32 antibody, GLYOXALASE I antibody, glyoxalase I homolog antibody, trypanothione-dependent glyoxalase I antibody, glyoxalase I antibody, glyoxalase 1 antibody, glyoxalase 1 L homeolog antibody, glyoxalase 1 S homeolog antibody, lactoylglutathione lyase antibody, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein antibody, GLO1 antibody, Glo1 antibody, glo1 antibody, glo1.L antibody, glo1.S antibody, GST3 antibody, GLX1 antibody, STY1687 antibody, Bcen_2094 antibody, gloA antibody

Background: The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Molecular Weight: 21 kDa

Gene ID: 2739

UniProt: Q04760