DPM1 antibody (Center)

Catalog No. ABIN2856272

Product Details anti-DPM1 Antibody

Target: DPM1 (Dolichyl-Phosphate Mannosyltransferase Polypeptide 1, Catalytic Subunit (DPM1))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPM1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Cow (Bovine), Pig (Porcine), Rat (Rattus), Xenopus laevis

Cross-Reactivity (Details): Mouse (90 %), Bovine (92 %), Pig (93 %), Rat (91 %), Xenopus laevis (86 %)

Characteristics: Rabbit Polyclonal antibody to DPM1 (dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit)
DPM1 antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DPM1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: NT2D1 , U87-MG , MCF-7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.95 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DPM1

Target: DPM1 (Dolichyl-Phosphate Mannosyltransferase Polypeptide 1, Catalytic Subunit (DPM1))

Alternative Name: DPM1 (DPM1 Products)

Synonyms: DPM1 antibody, zgc:101018 antibody, MGC114892 antibody, AI118379 antibody, AI194292 antibody, CDGIE antibody, MPDS antibody, DPMS antibody, dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit antibody, dolichyl-phosphate mannosyltransferase subunit 1, catalytic antibody, dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit S homeolog antibody, dolichol-phosphate (beta-D) mannosyltransferase 1 antibody, dpm1 antibody, DPM1 antibody, dpm1.S antibody, Dpm1 antibody

Background: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.

Cellular Localization: Endoplasmic reticulum

Molecular Weight: 30 kDa

Gene ID: 8813

Pathways: Inositol Metabolic Process