TUBB1 antibody

Catalog No. ABIN2856496

Product Details anti-TUBB1 Antibody

Target: TUBB1 (Tubulin, beta 1 (TUBB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to beta Tubulin (tubulin, beta 1)
beta Tubulin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , HepG2 , NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TUBB1

Target: TUBB1 (Tubulin, beta 1 (TUBB1))

Alternative Name: tubulin beta 1 class VI (TUBB1 Products)

Synonyms: TUBB3 antibody, 2810484G07Rik antibody, M(beta)1 antibody, TUBB1 antibody, tubulin beta 1 class VI antibody, tubulin, beta 1 class VI antibody, TUBB1 antibody, Tubb1 antibody

Background: Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288], Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

Molecular Weight: 50 kDa

Gene ID: 81027

UniProt: Q9H4B7

Pathways: Microtubule Dynamics