NDUFA5 antibody (full length)
-
- Target See all NDUFA5 Antibodies
- NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))
-
Binding Specificity
- full length
-
Reactivity
- Human, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This NDUFA5 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Chimpanzee
- Cross-Reactivity (Details)
- Chimpanzee (100 %)
- Characteristics
-
Rabbit Polyclonal antibody to NDUFA5 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13 kDa)
NDUFA5 antibody [N1C3] - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Full length human NDUFA5 Recombinant protein.
- Isotype
- IgG
- Top Product
- Discover our top product NDUFA5 Primary Antibody
-
-
- Application Notes
- Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Comment
-
Positive Control: H1299 , HeLa , HepG2 , mouse brain
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- Target
- NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))
- Alternative Name
- NDUFA5 (NDUFA5 Products)
- Synonyms
- NADHUO antibody, B13 antibody, CI-13KD-B antibody, CI-13kB antibody, NUFM antibody, UQOR13 antibody, 2900002J19Rik antibody, NADH:ubiquinone oxidoreductase subunit A5 antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 antibody, Ndufa5 antibody, NDUFA5 antibody
- Background
-
The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.
Cellular Localization: Mitochondrion inner membrane, Peripheral membrane protein, Matrix side - Molecular Weight
- 13 kDa
- Gene ID
- 4698
-