Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488)
| Antigen |
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| Synonyms |
ILRA, CD127, IL7RA, CDW127, IL-7R-alpha, IL7R |
| Clonality |
Monoclonal (A7R34) |
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Host
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Reactivity
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Conjugate
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FITC (9), Biotin (5), Alexa Fluor 488 (4), Alexa Fluor 647 (4), Alexa Fluor 350 (2), Alexa Fluor 546 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RPE (1)
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Application
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Western Blotting (WB) (34), Immunofluorescence (IF) (33), ELISA (30), Flow Cytometry (FACS) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (IHC) (6), Immunoprecipitation (IP) (6), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Neutralization (Neut) (2)
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| Catalog no. |
ABIN286590 |
| Quantity |
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| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD127
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Isotype
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IgG2a
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Clone
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A7R34
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Specificity
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The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL -7 receptor. IL -7 receptor alpha chain is expressed by immature B cells in the bone marrow, double -negative (CD4 -/CD8 -), single -positive (CD4+ and CD8+), but not double -positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL -7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL -7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127., Mouse
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Application Notes
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This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This A7R34 antibody has been reported for use in flow cytometric analysis.
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Buffer
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Phosphate buffer pH 7.2,150 mM NaCl, 0.09% NaN3, 0.1% gelatin
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Storage
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Store at 4 °C.DO NOT FREEZE.LIGHT -SENSITIVE MATERIAL.
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Restrictions
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For Research Use only
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Araki, Turner, Shaffer et al.: "mTOR regulates memory CD8 T-cell differentiation." in: Nature, Vol. 460, Issue 7251, pp. 108-12, 2009 (PubMed).
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Alternatives for antigen "Interleukin 7 Receptor (IL7R)", type "Antibodies"
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Hosts
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Rabbit (59), Mouse (16), Rat (13), Goat (2), Sheep (1)
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Reactivities
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Human (69), Mouse (Murine) (68), Rat (Rattus) (45), Horse (Equine) (36), Chicken (19), Cow (Bovine) (19), Dog (Canine) (19), Pig (Porcine) (18), Cat (Feline) (1)
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Applications
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Western Blotting (WB) (34), Immunofluorescence (IF) (33), ELISA (30), Flow Cytometry (FACS) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (IHC) (6), Immunoprecipitation (IP) (6), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Neutralization (Neut) (2)
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Conjugates
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FITC (9), Biotin (5), Alexa Fluor 488 (4), Alexa Fluor 647 (4), Alexa Fluor 350 (2), Alexa Fluor 546 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RPE (1)
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Epitopes
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pTyr449 (21), N-Term (20), AA 21-239 (2), C-Term (2), Extracellular Domain (1), Internal Region (1), pTyr496 (1)
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Alternatives 
