Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488)

Details for Product No. ABIN286590
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Antigen
Synonyms IL7R, CD127, IL-7Ralpha, CDW127, IL-7R-alpha, IL7RA, ILRA, IL-7RA
Reactivity
Mouse (Murine)
(117), (109), (34), (12), (12), (12), (12), (1)
Host
Rat
(87), (55), (33), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Alexa Fluor 488
(13), (13), (10), (8), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(87), (71), (56), (20), (15), (14), (10), (8), (6), (6), (4), (4), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 25 μg
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Catalog No. ABIN286590
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Clone A7R34
Isotype IgG2a
Specificity The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor.
Alternative Name CD127
Background IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-/CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Application Notes This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1 µg per million cells in a 100 µL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This A7R34 antibody has been reported for use in flow cytometric analysis.
Comment

Excitation/Emission wavelength: 495 nm/515 nm

Restrictions For Research Use only
Buffer Phosphate buffer pH 7.2,150 mM NaCl, 0.09 % NaN3, 0.1 % gelatin
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Supplier Images
anti-Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488) Staining of BALB/c splenocytes with APC anti -mouse CD3e (145 -2C11) (ABIN282622) and 0.5 µg of Alexa Fluor ® 488 Rat IgG2a Iso Cntrl (ABIN287234) (left) or 0.5 µg Alexa Fluor ® 488 anti -mouse CD127 (A7R34) (right). Cells in the lymphocyte gate were used for analysis.
anti-Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488) (2) anti-Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488) (Image 2)
General Araki, Turner, Shaffer et al.: "mTOR regulates memory CD8 T-cell differentiation." in: Nature, Vol. 460, Issue 7251, pp. 108-12, 2009 (PubMed).

Validation Images
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