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IL7R antibody (Interleukin 7 Receptor) Alexa Fluor 488

Details for Product anti-IL7R Antibody No. ABIN286590, Supplier: Login to see New
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Antigen
Alternatives
Reactivity
Mouse (Murine)
(180), (150), (29), (3)
Host
Rat
(102), (100), (69), (13), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
This IL7R antibody is conjugated to Alexa Fluor 488
(28), (21), (18), (17), (10), (10), (10), (10), (9), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(127), (96), (72), (27), (22), (16), (13), (12), (11), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Quantity 25 μg
Shipping to United States ( )
Clone A7R34
Isotype IgG2a kappa
Specificity The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Characteristics The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Synonyms: Synonyms: Interleukin-7 Receptor alpha
Alternative Name CD127 (IL7R Antibody Abstract)
Background IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-/CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Pathways JAK-STAT Signaling
Application Notes Applications reported: This A7R34 antibody has been reported for use in flow cytometric analysis.

Applications tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Comment

Excitation/Emission wavelength: 495 nm/515 nm

Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light sensitive material.
Storage 4 °C
Supplier Images
Image no. 1 for anti-IL7R antibody (Interleukin 7 Receptor)  Alexa Fluor 488 (ABIN286590) Staining of BALB/c splenocytes with APC anti -mouse CD3e (145 -2C11) (ABIN282622) and...
Image no. 2 for anti-IL7R antibody (Interleukin 7 Receptor)  Alexa Fluor 488 (ABIN286590) anti-Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488) (Image 2)
Background publications Araki, Turner, Shaffer et al.: "mTOR regulates memory CD8 T-cell differentiation." in: Nature, Vol. 460, Issue 7251, pp. 108-12, 2009 (PubMed).

Catalog No. ABIN286590
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