You are viewing an incomplete version of our website. Please click to reload the website as full version.

IL7R antibody (Interleukin 7 Receptor) (Alexa Fluor 488)

Details for Product anti-IL7R Antibody No. ABIN286590, Supplier: Log in to see
Antigen
Alternatives
anti-Mouse (Murine) IL7R antibody for Flow Cytometry
Reactivity
Mouse (Murine)
164
145
27
3
Host
Rat
93
93
67
12
2
Clonality (Clone)
Monoclonal ()
Conjugate
This IL7R antibody is conjugated to Alexa Fluor 488
25
20
17
16
10
10
9
9
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
118
89
64
24
22
13
12
12
11
9
5
4
4
4
3
3
3
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Clone A7R34
Isotype IgG2a kappa
Specificity The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Characteristics The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Synonyms: Synonyms: Interleukin-7 Receptor alpha
Alternative Name CD127 (IL7R Antibody Abstract)
Background IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-/CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+/CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Pathways JAK-STAT Signaling
Application Notes Applications reported: This A7R34 antibody has been reported for use in flow cytometric analysis.

Applications tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Comment

Excitation/Emission wavelength: 495 nm/515 nm

Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light sensitive material.
Storage 4 °C
Supplier Images
 image for anti-IL7R antibody (Interleukin 7 Receptor)  (Alexa Fluor 488) (ABIN286590) Staining of BALB/c splenocytes with APC anti -mouse CD3e (145 -2C11) (ABIN282622) and...
 image for anti-IL7R antibody (Interleukin 7 Receptor)  (Alexa Fluor 488) (ABIN286590) anti-Interleukin 7 Receptor (IL7R) antibody (Alexa Fluor 488) (Image 2)
Background publications Araki, Turner, Shaffer et al.: "mTOR regulates memory CD8 T-cell differentiation." in: Nature, Vol. 460, Issue 7251, pp. 108-12, 2009 (PubMed).