COL2 antibody

Catalog No. ABIN289403

Product Details anti-COL2 Antibody

Target: COL2 (Collagen, Type II (COL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: This lot of antisera reacts to both human and bovine PTH C-Terminal region.

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Cross-Reactivity (Details): This antibody that has been prepared bi immunoaffininty chromotography using immobilized antigens is followed by extensive cross-absorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins toremove any unwanted specifities.

Purification: Purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: Collagen type II antibody was raised in rabbit using collagen type II from human knee cartilage and bovine nasal cartilage as the immunogen.

Application Details

Application Notes: ELISA: 1:5,000-1:10,000, IHC: 1:50-1,200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator.

Comment:

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid In 0.125 M Na2B4O7, pH 8.0, with 0.075 M NaCl, 0.005 M EDTA, and 0.01 % Sodium azide.

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

References for anti-COL2 antibody (ABIN289403)

Murphy, Huey, Hu, Athanasiou: "TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Collagen Type II (COL2 Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, CAP18 antibody, Cnlp antibody, Cramp antibody, FALL39 antibody, MCLP antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, cathelicidin antimicrobial peptide antibody, Cuticle collagen 2 antibody, collagen type II alpha 1 chain antibody, F2 antibody, Camp antibody, col-2 antibody, COL2A1 antibody