Actin antibody

Antigen: Actin

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Clonality: Monoclonal (2G2)

Host: Mouse

Reactivity: Human, Cow (Bovine), Rabbit, Chicken, Xenopus laevis

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN289629

Additional Information

Immunogen: Profilin-Actin complex with poly A/U from calfthyme

Clone: 2G2

Description: Specific for skeletal and non-muscle actin isoforms (Mr 43,000). When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after Fixation with 4% paraformaldehyde. Immunostaining with cytoplasmatic actin was only positive after Fixation with methanol (Gonsior et al., 1999)

Specificity: Epitope location: N-V-P-A-M-Y-V-A-I-V-L-D-S-G-V-T-H-N-V-P-I-Y-H-A-I-M-R-L-D-L-A. Reactivities on Cultured Cell lines (tested so far): PtK2, SR-NRK, NRK-49F, L6 cells, rat fibroblasts, NIH-3T3, Mouse myoblast and myotube cells

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Application Details

Application Notes: With paraffin embedded sections, protease pretreatment is required prior to antibody application.

Storage: Tissue culture supernatant: -20

Images

Modelling of the 2G2 binding epitope: (A) Ribbon model of the actin molecule as generated from the data of Kabsch et al. (1990). Subdomain 1 is on the left. (B) Ribbon model as in A but with the three reactive sequences coloured (orange: aa131-139, yellow: aa155-169, green: aa176-187). (C) Model as in B, but coloured sequences in space filling mode, (D) Space filling model of the actin molecule with the reactive sequences coloured as in B.

SDS-PAGE and immunoblot analysis of anti-actin 2G2 reactivity with purified rabbit a-skeletal muscle actin (lane 1), cultured C2C12-myoblasts (lane 2), cultured C2C12-myotubes (lane3), isolated nuclei of C2C12-myoblasts after centrifugation over 2.12 M sucrose in buffer Lanes 1 - 4, Coomassie-blue stain, Lanes 1

Confocal sections through Rat2 fibroblasts (left) and HeLa cells (right) immunostained with 2G2 (C, D) are shown. Cells were extracted with 2% octyl-POE (n-octylpolyoxyethylene, Alexis) in modified Hanks

Publications

Publications: Gonsior, Platz, Buchmeier et al.: "Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody." in: Journal of cell science, Vol. 112 ( Pt 6), pp. 797-809, 1999 (PubMed).

Zhang, Buder, Burkhardt et al.: "Nuclear DNA helicase II/RNA helicase A binds to filamentous actin." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 843-53, 2002 (PubMed).

Peitsch, Hofmann, Endlich et al.: "Cell biological and biochemical characterization of drebrin complexes in mesangial cells and podocytes of renal glomeruli." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 6, pp. 1452-63, 2003 (PubMed).

Bettinger, Gilbert, Amberg: "Actin up in the nucleus." in: Nature reviews. Molecular cell biology, Vol. 5, Issue 5, pp. 410-5, 2004 (PubMed).