Immunoblot analysis of precipitates from skeletal muscle extracts. Metavinculin was precipitated from skeletal muscle extracts with the metavinculin-specific antibody 6E3. Untreated muscle lysate (CE, left lane), the metavinculin precipitate (6E3, center lane), and a control where the precipitating antibody had been omitted (right lane) were analyzed for the presence of both vinculin isoforms with the hVin1 antibody. Note that approximately equal amounts of Vinculin are coprecipitated with Metavinculin. Source: Comparative biochemical analysis suggests that vinculin and metavinculin cooperate in muscular adhesion sites Witt S, Zieseniss A, Fock U, Jockusch BM, Illenberger S. J Biol Chem. 2004 Jul 23,279(30):31533-43.
Localization of metavinculin in smooth muscle. Immunofluorescence images of cryosections of murine urinary bladder, as obtained with the metavinculin-specific antibody . Transversal (left) and longitudinal sections (right) are shown. Insets, higher magnifications of the boxed regions. Anti-MVT 6E3 react with specific dots (