AGEs antibody

Antigen: AGEs

Clonality: Monoclonal (6D12)

Host: Mouse

Reactivity: All Species

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN289930

Additional Information

Immunogen: AGEs-BSA

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6D12

Description: Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recent immunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modified proteinsin several human tissues: human lens (nondiabetic and noncataractous), renal proximal tubules inpatients with diabetic nephropathy and chronic renal failure, diabetic retina, peripheral nerves ofdiabetic neuropathy, atherosclerotic lesions of arterial walls, beta2-microglobulin forming amyloid fibrilsin patients with hemodialysis-related amyloidosis, senile plaques of patients with Alzheimer

Specificity: The initial study (Ref. 1) revealed that 6D12 does not recognize early products (Schiff base and Amadoriproducts), but shows a positive reaction to AGEs-samples obtained either from proteins, lysine derivatives ormonoamino-carboxylic acids, indicating the immunospecificity to a common structure among AGEs-structures.The subsequent study (Ref. 10) revealed of 6D12 is an N epsilon-carboxymethyllysine(CML)-protein adduct.

Application Details

Application Notes: Working dilution for immunohistochemistry: 2μg /mL; for ELISA: 0.1-0.5μg /mL; for WB : 0.25-5μg /mL

Concentration: 0.25 mg/ml

Purification: The splenic lymphocytes from BALB/c mouse, immunized with AGEs-BSA were fused to myeloma P3U1 cells. The hybrid cells were screened, and the cell line (6D12) with positive reaction to AGEs-human serum albumin but negative to BSA was selected through successive subclonings and grown in ascitic fluid of BALB/c mouse, from which the anti-AGEs antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below –20°C. Once thawed, store at –4°C. Repeated freeze-thaw cycles should be avoided.

Research Area: Immunology, Metabolism, Diabetes

Restrictions: For Research Use only

Images

Immunohistochemical staining of renal proximal tubules andglomeruli in patients with diabetic nephropathy, using anti-AGEs antibody 6D12 (Yamada, K. et al,.Clinical nephrology, Vol.42, 354-361, 1994)

Publications

Publications: Nagai, Fujiwara, Mera et al.: "Usefulness of antibodies for evaluating the biological significance of AGEs." in: Annals of the New York Academy of Sciences, Vol. 1126, pp. 38-41, 2008 (PubMed).

McRobert, Tikoo, Cooper et al.: "Localization of the ezrin binding epitope for advanced glycation endproducts." in: The international journal of biochemistry & cell biology, Vol. 40, Issue 8, pp. 1570-80, 2008 (PubMed).

Li, Ota, Nakamura et al.: "Antiglycation effect of gliclazide on in vitro AGE formation from glucose and methylglyoxal." in: Experimental biology and medicine (Maywood, N.J.), Vol. 233, Issue 2, pp. 176-9, 2008 (PubMed).

Nagai, Fujiwara, Mera et al.: "Immunochemical detection of Nepsilon-(carboxyethyl)lysine using a specific antibody." in: Journal of immunological methods, Vol. 332, Issue 1-2, pp. 112-20, 2008 (PubMed).

Mera, Nagai, Brock et al.: "Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products." in: Journal of immunological methods, Vol. 334, Issue 1-2, pp. 82-90, 2008 (PubMed).

Diamanti-Kandarakis, Katsikis, Piperi et al.: "Increased serum advanced glycation end-products is a distinct finding in lean women with polycystic ovary syndrome (PCOS)." in: Clinical endocrinology, Vol. 69, Issue 4, pp. 634-41, 2008 (PubMed).

Unterluggauer, Micutkova, Lindner et al.: "Identification of Hsc70 as target for AGE modification in senescent human fibroblasts." in: Biogerontology, Vol. 10, Issue 3, pp. 299-309, 2009 (PubMed).

Sohn, Kim, Kim et al.: "KIOM-79 prevents apoptotic cell death and AGEs accumulation in retinas of diabetic db/db mice." in: Journal of ethnopharmacology, Vol. 121, Issue 1, pp. 171-4, 2009 (PubMed).

Wei, Chen, Chen et al.: "Rapid glycation with D-ribose induces globular amyloid-like aggregations of BSA with high cytotoxicity to SH-SY5Y cells." in: BMC cell biology, Vol. 10, pp. 10, 2009 (PubMed).

Rabbani, Thornalley: "Quantitation of markers of protein damage by glycation, oxidation, and nitration in peritoneal dialysis." in: Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis, Vol. 29 Suppl 2, pp. S51-6, 2009 (PubMed).

Chen, Wei, Wang et al.: "D-Ribosylated Tau forms globular aggregates with high cytotoxicity." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 15, pp. 2559-71, 2009 (PubMed).

Davies, Herrick, Cordingley et al.: "Expression of advanced glycation end products and their receptor in skin from patients with systemic sclerosis with and without calcinosis." in: Rheumatology (Oxford, England), Vol. 48, Issue 8, pp. 876-82, 2009 (PubMed).

Joshi, Gupta, Dubey et al.: "TRC4186, a novel AGE-breaker, improves diabetic cardiomyopathy and nephropathy in Ob-ZSF1 model of type 2 diabetes." in: Journal of cardiovascular pharmacology, Vol. 54, Issue 1, pp. 72-81, 2009 (PubMed).

Kim, Yi, Kim et al.: "Novel application of surface plasmon resonance biosensor chips for measurement of advanced glycation end products in serum of Zucker diabetic fatty rats." in: Biosensors & bioelectronics, Vol. 25, Issue 1, pp. 248-52, 2009 (PubMed).