AGEs antibody (Biotin)
| Antigen |
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| Clonality |
Monoclonal (6D12) |
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Host
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Reactivity
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Conjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Peroxidase (POD) (1), RBITC (1)
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Application
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| Catalog no. |
ABIN289931 |
| Quantity |
10 ug (0.25 mg/ml) |
| Price |
1,142.89 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Immunogen
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AGEs-BSA
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Format
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Affinity-purified
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Isotype
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IgG1
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Clone
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6D12
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Description
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Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recentimmunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modified proteinsin several human tissues: human lens (nondiabetic and noncataractous), renal proximal tubules inpatients with diabetic nephropathy and chronic renal failure, diabetic retina, peripheral nerves ofdiabetic neuropathy, atherosclerotic lesions of arterial walls, beta2-microglobulin forming amyloid fibrilsin patients with hemodialysis-related amyloidosis, senile plaques of patients with Alzheimer
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Specificity
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The initial study (Ref. 1) revealed that 6D12 does not recognize early products (Schiff base and Amadoriproducts), but shows a positive reaction to AGEs-samples obtained either from proteins, lysine derivatives ormonoamino-carboxylic acids, indicating the immunospecificity to a common structure among AGEs-structures.The subsequent study (Ref. 10) revealed of 6D12 is an Nepsilon- carboxymethyllysine(CML)-protein adduct.
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Application Notes
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Working dilution for immunohistochemistry: 2ug /mL, for ELISA: 0.1-0.5ug /mL, for WB : 0.25-5ug /mL
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Concentration
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0.25 mg/ml
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Purification
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The splenic lymphocytes from BALB/c mouse, immunized with AGEs-BSA were fused to myeloma P3U1 cells. The hybrid cells were screened, and the cell line(6D12) with positive reaction to AGEs-human serum albumin but negative to BSAwas selected through successive subclonings and grown in ascitic fluid of BALB/cmouse, from which the anti-AGEs antibody was purified by Protein G affinity chromatography (Reference No.1) and conjugated.
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Buffer
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PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)
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Storage
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Store below -20
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Restrictions
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For Research Use only
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Alternatives for antigen "AGEs", type "Antibodies"
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Hosts
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Rabbit (20), Mouse (2)
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Reactivities
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Human (19), Mouse (Murine) (15), Rat (Rattus) (15), All Species (2)
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Applications
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Immunofluorescence (IF) (18), ELISA (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Western Blotting (WB) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Peroxidase (POD) (1), RBITC (1)
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Alternatives 
