Macrophage/Dendritic Cells antibody

Antigen: Macrophage/Dendritic Cells

Clonality: Monoclonal (RM-4)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN289947

Additional Information

Immunogen: homogenate of silica-induced lung granuloma of rat

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: RM-4

Description: This anti-rat macrophage/dendritic cells monoclonal antibody, RM-4, was produced by using ahomogenate of silica-induced lung granulomas of rat as immunogen. Immunohistochemistry demonstratedthat RM-4 is specific for rat macrophages and dendritic cell populations in various organs and tissues.In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigen, ofwhich molecular weight is 46kDa, presenting on the membrane of endosomes and lysosomes inmacrophages and dendrictic cells.Thus, this antibody is very useful for not only in discriminating macrophage/dendritic cells from variouscell populations but also in identifying endosomes and lysosomes.

Application Details

Application Notes: Working dilution for immunohistochemistry: 10ug/ml on frozen sections and paraffin sections.

Concentration: 0.25 mg/ml

Purification: The splenic lymphocytes from BALB/c mouse, immunized with homogenate of silica-induced lung granuloma of rat, were fused to myeloma NS-1 cells. Thescreening of the hybridoma cells was performed on cryostat sections of ratspleens. The hybridoma cell line (RM-4) with positive reaction was grown inascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Rat liver (frozen section): Kupffer cells are positively stained (Takeya M.,Second Department of Pathology, KumamotoUniversity School of Medicine, Kumamoto, Japan)

Rat spleen (frozen section): Macrophages in red pulp and white pulp were positivery stained. Takeya M.,Second Department of Pathology, KumamotoUniversity School of Medicine, Kumamoto, Japan

Specificity by organ