Macrophage/Monocyte antibody

Antigen: Macrophage/Monocyte

Clonality: Monoclonal (RbM2)

Host: Mouse

Reactivity: Rabbit

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN289948

Additional Information

Immunogen: thioglycate-elicited rabbit peritoneal macrophages

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: RbM2

Description: This anti-rabbit macrophage monoclonal antibody, RbM2, was produced by using thiogllycate-elicitedrabbit peritoneal macrophages as immunogen. Immunoelectoron microscopy demonstrates that RbM2reacts with lysosomes of rabbit macrophages and monocytes. This selective reactivity was confirmed invarious experiments by endocytosis. In contrast, dendritic cells, such as follicular dendritic cells (FDCs) oflymphoid follicles, interdigitating cells(IDCs) of lymphoid T zone, or epidermal Langerhan

Application Details

Application Notes: Working dilution for immunohistochemistry: 10ug/ml on frozen sections, not applicable for paraffin sections.

Concentration: 0.25 mg/ml

Purification: The splenic lymphocytes from BALB/c mouse, immunized with thioglycate-elicitedrabbit peritoneal macrophages, were fused to myeloma NS-1 cells. The screening ofthe hybridoma cells was performed on cryostat sections of rabbit spleens. Thehybridoma cell line (RbM2) with positive reaction was grown in ascitic fluid ofBALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Rabbit Liver (frozen section): Kupffer cells are positively stained. (Takeya M., Second Department of Pathology,Kumamoto University School of Medicine, Kumamoto, Japan)

Specificity by organ