Macrophage Scavenger Receptor A (MSRA) antibody

Antigen: Macrophage Scavenger Receptor A (MSRA)

Clonality: Monoclonal (SRA-E5)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN289954

Additional Information

Alternative name: Macrophage Scavenger Receptor A(MSR-A:CD204)

Immunogen: recombinant protein of human type I SR-A

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: SRA-E5

Description: Macrophage scavenger receptor (MSR-A: CD204) was identified in the search for the receptor molecules thatare implicated in the pathological deposition of cholesterol during atherogenesis through receptor-mediateduptake of modified low density lipoproteins (LDL).MSR-As possess a wide range of ligand-binding specificities and recognize a variety of molecules such asmodified LDL including acetylated LDL, oxidized LDL, advanced glycation end products (AGE),polyribonucleotides such as poly G and poly I and bacterial surface lipids including lipopolysaccharide andlipoteicoic acid.This antidody was produced from the mouse immunized with recombinant protein of human type IMSR-A ,and has been proved to be useful for the immunoblotting and immunohistochemistry.This antibody is useful tools for the study of MSR in atherogenesis and various other pathological conditionsin humans and animal species.

Application Details

Application Notes: Working dilution for immunohistochemistry: 10ug/ml , for immunoblotting : 2ug/mlAntigen retrieval (microwave 10 min , 0.01M citrate buffer, ph2.0) is recommended for paraffin sections.

Concentration: 0.25 mg/ml

Purification: The spleen cells from mouse, immunized with recombinant protein of human type ISR-A, were fused with mouse NS-1 myeloma cells. The hybridoma cell line withpositive reaction was grown in ascitic fluid of BALB/c mouse, from which theantibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Skin Sarcoidosis (paraffin section). Epithelioid cells and multi-nucleatedgiant cells in granulomas andsurrounding macrophages are positive. (Takeya M., Second Department of Pathology, Kumamoto University School of Medicine, Kumamoto, Japan)

Human liver (paraffin section) Kupffer cells are positively stained.

Specificity by organ