Nitrotyrosine antibody

Antigen: Nitrotyrosine

Clonality: Monoclonal (2H1)

Host: Mouse

Catalog no.: ABIN289971

Additional Information

Immunogen: Nitrotyrosine-HSA

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2H1

Description: To elucidate the function of Nitric Oxide (NO) related signal transduction, we developed a new monoclonal antibody to Nitrotyosin (Clone No.2H1). There are two pathways, which isengaged in the signal transduction regarding vascular relaxation on endothelial cell. One isthrough activated guanlyate cyclase that is cGMP dependent and another is cGMPindependent pathway which requires reactive NO derived substance such as,peroxinitrate (ONOO), N2O3,N2O4(NO2).Recently, various functions of reactive NO derived substance has been identified forexample regulating protein phosphorylation and inducing apoptosis. So, it is believed thatreactive NO derived substance is involved in signal transduction among cells.Immunohystochemical study revealed that nitotyrosin residue is produced in some patientsuch as athrosclerosis, Alzheimer

Application Details

Concentration: 0.25 mg/ml

Purification: The spleen cells from BALB/c mouse, immunized with nitrotyrosin-HSA, were fused tomyeloma P3U1 cells. The screening of the hybridoma cells was performed on ELISA.The cell line was grown on non-serum medium, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 0.1% BSA as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only