Nephronectin (NPNT) antibody

Antigen: Nephronectin (NPNT)

Synonyms: POEM, EGFL6L, Nctn, AA682063, AI314031, 1110009H02Rik, si:ch211-202n8.2, egfl6l, DKFZp469K0816

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN289986

Additional Information

Alternative name: POEM/nephronectin

Immunogen: Partial peptide of Mouse POEM/nephronectin

Format: Affinity-purified

Description: POEM (Pre osteoblastic EGF repeat protein with MAM domain)/nephronectin is a novel ligand for alpha8beta1integrin. Its mRNA and protein is widely expressed in developing embryo, such as kidney, bone, musclesand endocrine organs except uriniferous tubule after childbirth. POEM/nephronectin estimated to encode a60k dalton protein which has several functional domains, such as a signal sequence, five EGF-like repeatedsequences, a proline-rich domain, a RGD cell binding motif and a MAM domain. POEM/nephronectinprotein is firmly bound to the cell surface after its secretion and not observed in culture medium. Recentresearch results indicate that not only the MAM domain but also the RGD cell binding motif inPOEM/nephronectin protein plays an important role in cell adhesion, spreading, and survival.This antibody was established from the purified serum immunized with a short peptide of mousePOEM/nephronectin. It is useful for immunohistochemical detection of POEM/nephronectin.

Application Details

Application Notes: Working dilution For Immunohistochemistry , 0.2-0.5 ug/ml

Concentration: 0.25 mg/ml

Purification: This antibody was purified from rabbit serum by affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Immunohistochemistry Sample: Mouse Kidney left, Anti POEM/nephronectin. Antibody right, control Rabbit IgG. Preparation of antibodies and instruction: Drs. Tezuka K. at Department of Tissue and Organ Development, Regenerationand Advanced Medical Science, Gifu University Graduate School of Medicine

Publications

Publications: Brandenberger, Schmidt, Linton et al.: "Identification and characterization of a novel extracellular matrix protein nephronectin that is associated with integrin alpha8beta1 in the embryonic kidney." in: The Journal of cell biology, Vol. 154, Issue 2, pp. 447-58, 2001 (PubMed).

Morimura, Tezuka, Watanabe et al.: "Molecular cloning of POEM: a novel adhesion molecule that interacts with alpha8beta1 integrin." in: The Journal of biological chemistry, Vol. 276, Issue 45, pp. 42172-81, 2001 (PubMed).