Phototropin 2 antibody

Antigen: Phototropin 2

Synonyms: K21L19.6, K21L19_6, NON PHOTOTROPIC HYPOCOTYL 1-LIKE, NPL1, PHOT2, PHOTOTROPIN 2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis

Application: Western Blotting (WB)

Catalog no.: ABIN290022

Additional Information

Immunogen: Partial peptide of Arabidopsis Phototropin 2

Format: Affinity-purified

Application Details

Application Notes: Working dilution For western blotting ,0.1-0.3ug/ml

Concentration: 0.25 mg/ml

Purification: This antibody was purified from rabbit serum immunized with partial peptide ofArabidopsis PHOT2 by peptide affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Western blotting: Sample: Arabidopsis leaves (1) Mutant type lacking PHOT2 (2) Hetero type (3) Wild type

Sample: GST fusion protein , expressed in Escherichia coli (1) GST-PHOT1 N terminal (without IPTG induction) (2) GST-PHOT1 N terminal (with IPTG induction) (3) GST-PHOT2 N terminal (without IPTG induction) (4) GST-PHOT2 N terminal (with IPTG induction). Preparation of antibodies and instruction: Higashi T, Kagawa T, Kimura MLife Science and Bioengineering,Graduate School of Life and Environmental Science,University of Tsukuba