CD68 Molecule (CD68) antibody

Antigen: CD68 Molecule (CD68)

Clonality: Monoclonal (PM-1K)

Host: Mouse

Reactivity: Human, Guinea Pig, Pig (Porcine), Cow (Bovine), Monkey

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN290048

Additional Information

Alternative name: Macrophage (CD68)

Immunogen: human peritoneal cells from patients with endometriosis incubated for 24 hours

Format: Affinity-purified

Isotype: IgG2b, kappa chain  (Matching secondary antibodies)

Clone: PM-1K

Description: Macrophages are present in nearly all tissues and organs of the body. They are differentiated from monocytesderived from the bone marrow. Macrophages and monocytes are phagocytes, acting in both innate immunity andcell-mediated immunity of vertebrate animals. Their function is to phagocytize cellular debris and pathogens,and to stimulate lymphocytes and other immune cells to respond to the pathogen.Macrophages are able to be identified immnohistochemically by virtue of the presence ofmonocyte/macrophage-associated antigens such as CD68. Antibodies recognizing CD68 have been used as someof the best reagents to detect macrophages in tissues.CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissuemacrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family.This antibody recognizes CD68 antigen. The molecular size of the antigen identified by this antibody was110 kDa. Immunopecipitated antigen by this antibody was also recognized by the other CD68 antibodies such asKP-1 and PG-M1.In immunohistochemical assays, this antibody recognizes freshly isolated human blood monocytes andtissue macrophages. This antibody also recognizes macrophages obtained from guinea pigs, pigs, bovine species,and monkeys. Since this antibody strongly labels guinea pig macrophages, this antibody will be suitable toexamine such macrophages in experimental guinea pig models.This antibody will be very useful to research of CD68, macrophage, allergic diseases and delayedhypersensitivity.

Application Details

Application Notes: Working dilution for immunohistochemistry: 10ug/ml, Please observe that this AB is not suitable for paraffin sample sections in tissues from all species (please inquire). Frozen sections are recommended.

Concentration: 0.25 mg/ml

Purification: The splenic lymphocytes from BALB/c mouse, immunized with human peritoneal cells from patients with endometriosis incubated for 24 hours, were fused withmouse NS-1 myeloma cells. The hybridoma cell line with positive reaction was grown inascitic fluid of BALB/c mouse, from which the antibody was purified by Protein Gaffinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Human spleen (frozen section): Red pulpmacrophages are positive.

Small Intestine of Guinea Pig (paraffin section): Macrophages in lamina propria arepositive. Takeya M., Department of Cell Pathology,Graduate School of Medical Sciences,Kumamoto University, Kumamoto, Japan

Publications

Publications: Horikawa, Komohara, Kiyota et al.: "Detection of guinea pig macrophages by a new CD68 monoclonal antibody, PM-1K." in: Journal of molecular histology, Vol. 37, Issue 1-2, pp. 15-25, 2006 (PubMed).