MSR- A:CD204 antibody

Antigen: MSR- A:CD204

Clonality: Monoclonal (SRA-C6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB), Neutralization (Neut)

Catalog no.: ABIN290049

Additional Information

Immunogen: recombinant protein of Human MSR-A(CD204)

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: SRA-C6

Description: Class A macrophage scavenger receptor (MSR-A: CD204) was identified in the search for the receptor molecules that are implicated in the pathological deposition of cholesterol during atherogenesis through receptor-mediated uptake of modified low density lipoprotein (LDL). MSR-A possesses a wide range of ligand-binding specificities and recognize a variety of molecules such as modified LDL including acetylated LDL, oxidized LDL, advanced glycation end products (AGEs), polyribonucleotides such as poly G and poly I and bacterial surface lipids including lipopolysaccharide and lipoteicoic acid.This antidody was produced from the mouse immunized with recombinant protein of human type I MSR-A ,and has been proved to be useful for the western blotting and immunohistochemistry. This antibody also inhibits the endocytic degradation of acetylated LDL and oxidized LDL by high glucose-treated human monocyte-derived macrophages and has anti MSR-A neutralizing activity.This antibody is useful tools for the study of MSR-A in atherogenesis and various other pathological conditions.

Application Details

Application Notes: Working dilution for immunohistochemistry: 5.0ug/ml, western blotting : 1.0ug/ml,Neutralization: Depends on the experimental design (Application Reference:1)

Concentration: 0.25 mg/ml

Purification: The spleen cells obtained from MSR-A deficient mouse, immunized with recombinant protein corresponding to amino acid 131-451 of human type I MSR-A, were fused with mouse NS-1 myeloma cells. The hybridoma cell line with positive reaction was grown in ascitic fluid of BALB/c mouse, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Human alveolar macrophages (Cytospin preparation): Most macrophages are positive.

Human liver (paraffin section): Kupffer cells are positive

Neutralizing activity of SRA-C6 (20ug/ml): Inhibitory effect of anti-human SR-A antibody on the degradation of 125I-AcLDL by human monocyte-derived macrophages (day7)