Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4)

Synonyms: VRL2, TRP12, VRL-2, VROAC, OTRPC4, VR-OAC, TRPV4, wu:fp52e02, Trp12, MGC150050, 0610033B08Rik, Vroac, Otrpc4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN290050

Additional Information

Alternative name: TRPV4

Immunogen: Partial peptide of Mouse TRPV4

Format: Affinity-purified

Description: TRP (transient receptor potential) channels comprise a superfamily of non-selective cation channels with atleast nine subfamilies. The variety of subfamilies corresponds to the differences in the activation mechanismsand functions.TRPV4 (TRP vanilloid 4), first identified as an osmosensory ion channel, can also be activated by warmtemperatures (> 27-35 degrees C). TRPV4 is expressed in a wide variety of tissues (sensory neurons,hypothalamus, skin, kidney, lung, inner ear). TRPV4 is a primary afferent transducer that plays a crucial role inneuropathic hyperalgesia for osmotic and mechanical stimuli, as well as in inflammatory mediator-inducedhyperalgesia for osmotic stimuli. It functions as a Ca2+ entry channel and can be activated by a wide range ofstimuli including physical (cell swelling, heat, mechanical stimulation) and chemical stimuli (endocannabinoids,arachidonic acid metabolites, and 4alpha-phorbol esters). Moreover, TRPV4 plays a major role in mechanicalhyperalgesia and enhanced nociception to hypo-asmotic stimuli by Taxol.Given its wide expression and the variety of activatory stimuli, TRPV4 is likely to play a number ofphysiological roles. Studies with TRPV4(-/-) mice suggest a role for the channel in the regulation of bodyosmolarity, mechanosensation, and temperature sensing.This antibody will be very useful to research osmotic, mechanical stimuli, inflammatory reaction andthermosensitive response.

Application Details

Application Notes: Working dilution For Western blotting 0.25 ug/ml

Concentration: 0.25 mg/ml

Purification: This antibody was established from the serum of a rabbit immunized with the partial peptide corresponding to amino acid 3-17 of mouse TRPV4, and purified by peptideaffinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Western blotting: Lane 1: choroid plexus (Wild type mouse) Lane 2: choroid plexus (TRPV4 knockout mouse) Lane 3: rat TRPV4 overexpressed in HEK293 cells. Preparation of antibodies and instruction: Dr. Makoto Tominaga at Section of Cell Signaling,Okazaki Institute for Integrative Bioscience,National Institutes of Natural Sciences