AGE-3 antibody

Antigen: AGE-3

Clonality: Monoclonal (9D8)

Host: Mouse

Reactivity: All Species

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN290053

Additional Information

Immunogen: AGE3(glycolaldehyde-modified BSA)

Format: Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9D8

Description: The products of the nonenzymatic glycation and oxidation of proteins, lipids and nucleic acids, the advancedglycation end-products (AGEs), accumulate in various pathological conditions, such as diabetes, inflammation,renal failure, and aging. AGEs accumulate at site of microvascular injury in diabetes, including the kidney, theretina, and within the vasculature. The enhanced formation of AGEs also exists in various disease, such asatherosclerosis, Alzheimer

Application Details

Application Notes: Working dilution For Western blotting 1.0 ug/ml. For ELISA 0.1 ug/ml

Concentration: 0.25 mg/ml

Purification: The spleen cells from GANP mouse, immunized with glycolaldehyde-modified BSA(AGE-3), were fused to myeloma P3U1 cells. The screening of the hybridoma cellswas performed on ELISA. The cell line with positive reaction was grown on non-serummedium, from which the antibody was purified by Protein G affinity chromatography.

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Storage: Store below -20

Restrictions: For Research Use only

Images

Western blotting (1) AGE1-BSA (glucose-modified) (2) AGE2-BSA (glyceraldehyde-modified) (3) AGE3-BSA (glycolaldehyde-modified) (4) AGE4-BSA (methylglyoxal-modified) (5) AGE5-BSA (glyoxal-modified) (6) AGE6-BSA (3-DG-modified) (7) CML-BSA (carboxymethyllysine-modified) (8) CEL-BSA (carboxyethyllysine-modified) (9) BSA