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ATPase, Na+/K+ Transporting, beta 3 Polypeptide (ATP1B3) antibody

Antigen

ATPase, Na+/K+ Transporting, beta 3 Polypeptide (ATP1B3)

Synonyms CD298, ATPB-3, FLJ29027
Clonality Monoclonal (5G10)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Catalog no. ABIN290059
Quantity 50 ug  (0.25 mg/ml)
Price 942.65 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Alternative name CD298 (Na/K-ATPase b3)
Immunogen human cancer cel line
Format Affinity-purified
Isotype IgG2a kappa  (Matching secondary antibodies)
Clone 5G10
Description Na/K-ATPase is a membrane-associated enzyme responsible for the active transport of Na + and K+, and the maintenance of Na+ and K+ gradients across membrane. It is a heterodimer of two different kinds subunit, a large alpha subunit and a smaller glycoprotein subunit beta. At least four distinct alpha subunits and four beta subunit isoforms have been identified. In some tissues, it has been showed that Na/K-ATPase is associated with third subunit gamma, a small regulatory protein. CD298 (also known as Na/K-ATPase beta3) is a 42-kDa glycoprotein and one of beta subunit isoforms. CD298 has been detected in amphibians, mouse, rat,and human. In rat, CD298 expresses in a variety of tissues including testis, brain, kidney, spleen, stomach, small intestine, colon, lung, and liver. In theretina Na/K-ATPase restores Na+ and K+ gradients used by photoreceptor dark current, synaptic activity, action potential, and CD298 is largely restricted to photoreceptors in the mouse and rat. In human, CD298 also involves in regulation of T and B lymphocyte proliferation.This antibody is specific to human CD298 and will be useful for FCM, immunoprecipitation, immunohistochemistry.

Application Details

Application Notes WB Not tested. IHC 5.0-10.0 ug/ml. ICC Not tested. ELISA Not tested. FCM 0.5-1.0 ug/ml. Neutralization Not tested. IP 5.0-10.0 ug/ml
Concentration 0.25 mg/ml
Purification Protein G
Buffer PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)
Storage Store below -20
Restrictions For Research Use only

Alternatives

Alternatives for antigen "ATPase, Na+/K+ Transporting, beta 3 Polypeptide (ATP1B3)", type "Antibodies"
Hosts Mouse (10), Rabbit (2)
Reactivities Human (12)
Applications Enzyme Immunoassay (EIA) (5), Western Blotting (WB) (5), ELISA (Detection) (3), ELISA (2), Flow Cytometry (FACS) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)