Myc Tag (AA 423-435) antibody (Alkaline Phosphatase (AP))

Details for Product No. ABIN290539, Supplier: Log in to see
Antigen
Epitope
AA 423-435
56
9
6
4
3
2
2
2
2
2
1
1
Reactivity
Tag
212
36
14
10
6
5
3
1
1
Host
Mouse
160
60
17
16
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Alkaline Phosphatase (AP)
27
23
14
9
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
215
115
78
53
48
34
22
18
18
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
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Immunogen Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Monkey, Marmoset, Tamarin (92%), Rat, Sheep, Hamster, Panda, Dog, Horse (85%).

Type of Immunogen: Synthetic peptide
Clone 9E10
Isotype IgG1
Specificity Detects the 62kD c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. May also be used to detect the commonly used c-myc tag.
Predicted Reactivity Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Orangutan, Monkey, Marmoset, Tamarin (92%) Rat, Sheep, Hamster, Panda, Dog, Horse (85%).
Purification Affinity purified
Target Type Tag
UniProt P01106
Application Notes Approved: ELISA (1:500 - 1:5000), IHC-Fr, IHC-P, WB (1:100 - 1:500)

Usage: Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot: LS-C57629 detects a band of approximately 62kD in Colo 320HSR cell lysates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.4, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freezing
Storage 4 °C
Storage Comment Store at +4°C. DO NOT FREEZE.
Supplier Images
 image for anti-Myc Tag (AA 423-435) antibody (Alkaline Phosphatase (AP)) (ABIN290539) anti-Myc Tag (AA 423-435) antibody (Alkaline Phosphatase (AP))