Orexin antibody (AA 2-14)

Catalog No. ABIN291715

Product Details anti-Orexin Antibody

Target: Orexin (OX)

Binding Specificity: AA 2-14

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Orexin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Human orexin A, an excitatory neuropeptide linked to alertness and wakefulness in humans.

Purification: Affinity purified

Immunogen: Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 mg/mL. Histology Positive Control Tissue: Hypothalamus.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Orexin

Target: Orexin (OX)

Alternative Name: HCRT / Orexin (OX Products)

Background: Name/Gene ID: HCRT

Synonyms: HCRT, PPORX, OX, Hypocretin, NRCLP1, Orexin

Gene ID: 3060

UniProt: O43612

Pathways: Synaptic Membrane, Feeding Behaviour