Versican antibody (AA 1344-1554)
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- Target See all Versican (Vcan) Antibodies
- Versican (Vcan)
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Binding Specificity
- AA 1344-1554
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Versican antibody is un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA
- Specificity
- Recognizes human Versican. Species sequence Homology: For immunizing sequence, aa1344-1554: canine (80 % ), mouse (60 %).
- Purification
- Immunoaffinity purified
- Immunogen
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Recombinant protein corresponding to aa1344-1554 from human Versican isoform V0, expressed in E. coli (P13611).
Type of Immunogen: Recombinant protein - Isotype
- IgG
- Top Product
- Discover our top product Vcan Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhVersican is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects Versican in Hela cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL detects Versican in cryosections of E13.5 mouse embryos. Tissue was fixed with PBS containing 4 %PFA for 20 min. at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton and 1 % BSA for 45 minutes at RT. After blocking, sections were incubated with diluted primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL. Detection limit for rhVersican is ~0.1 ng/well. Optimal dilutions should be determined by each laboratory. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose.
- Handling Advice
- Avoid frequent freeze thaws. Store undiluted.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -70°C. Stable for 1 year from date of receipt.
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- Target
- Versican (Vcan)
- Alternative Name
- VCAN / Versican (Vcan Products)
- Synonyms
- br146 antibody, cspg2 antibody, cspg2a antibody, sb:cb63 antibody, versican antibody, CSPG2 antibody, Cspg2 antibody, wgn antibody, ervr antibody, pg-m antibody, wgn1 antibody, Xversican antibody, dcan antibody, cspg2b antibody, fb99b07 antibody, wu:fb99b07 antibody, ERVR antibody, GHAP antibody, PG-M antibody, WGN antibody, WGN1 antibody, 5430420N07Rik antibody, 9430051N09 antibody, DPEAAE antibody, NG2 antibody, PG-M(V0) antibody, PG-M(V1) antibody, hdf antibody, versican a antibody, versican antibody, versican b antibody, vcana antibody, VCAN antibody, Vcan antibody, vcan antibody, vcanb antibody
- Background
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Name/Gene ID: VCAN
Synonyms: VCAN, CSPG2, ERVR, GHAP, Large fibroblast proteoglycan, PG-M, Versican, WGN, Versican core protein, Versican proteoglycan, WGN1 - Gene ID
- 1462
- UniProt
- P13611
- Pathways
- Glycosaminoglycan Metabolic Process
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