His Tag antibody

Catalog No. ABIN294561

Product Details anti-His Tag Antibody

Target: His Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: HHHHHH

Specificity: The 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells

Purification: Affinity purified

Immunogen: 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:40000), WB (1:1000 - 1:3000)

Usage: This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles. Store undiluted.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

Target Details for His Tag

Target: His Tag

Abstract: His Tag Products

Target Type: Tag