IL-1 beta antibody (Biotin)

Catalog No. ABIN295086

Product Details anti-IL-1 beta Antibody (Biotin)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Recognizes canine Interleukin 1 beta (IL-1b).

Purification: Affinity purified

Immunogen: E. coli-derived recombinant canine IL-1beta.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: Flo, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Flow Cytometry and Western Blot. Western Blot: 0.1-0.2 μg/mL. Detection limit for recombinant canine IL-1beta is ~0.5 ng/lane under non-reducing and reducing conditions. Shows ~40 % cross-reactivity with recombinant feline IL-1beta, 10 % cross-reactivity with recombinant human IL-beta, rrmIL-1beta, recombinant equine IL-1 beta, recombinant porcine IL-1beta, and 5 % cross-reactivity with rmIL-1beta, recombinant rat IL-1beta, and rcrIL-1beta. Flow Cytometry: Dilute to 50 μg/mL. Use 10 μL of dilution to 1-2.5x10^5 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, sterile 40-50 % glycerol, TBS (20 mM Trizma base, 150 mM sodium chloride), 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized from PBS, BSA, labeled, biotin. Reconstitute, sterile 40-50 % glycerol, TBS (20 mM Trizma base, 150 mM sodium chloride), 0.1 % BSA.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B (IL1B Products)

Synonyms: IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody

Background: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome