LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 257-270)

Details for Product anti-LEF1 Antibody No. ABIN297259, Supplier: Log in to see
Antigen
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer-binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • lef1
  • Lef1
Alternatives
anti-Human LEF1 antibody for Enzyme Immunoassay
Epitope
AA 257-270
15
13
13
9
8
6
6
6
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish
129
40
30
10
7
6
4
4
4
4
3
3
2
1
1
Host
Goat
58
52
15
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
ELISA, Western Blotting (WB)
115
66
22
18
10
7
5
3
2
2
2
1
1
1
1
Options
Supplier
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Immunogen Synthetic peptide C-QHEQRKEQEPKRPH from an internal region of human LEF1 (NP_057353.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%), Xenopus (93%), Pufferfish (86%).

Type of Immunogen: Synthetic peptide
Specificity Human LEF1.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%) Xenopus (93%) Pufferfish (86%).
Purification Immunoaffinity purified
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA
Gene ID 51176
NCBI Accession NP_057353
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers, Wnt Signaling
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
Application Notes Approved: ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). Recommended concentration: 1-3 μg/mL.

Not recommended for: IHC-P
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. Minimize freezing and thawing.
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