Sca-1/Ly-6A/E antibody (FITC)

Catalog No. ABIN299007

Product Details anti-Sca-1/Ly-6A/E Antibody (FITC)

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes members of the lymphocyte activation protein-6 (Ly6) multigene family. Specifically recognizes Ly6A.2 and Ly6E.1. Ly6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kD mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells. Reported to induce T cell activation.

Purification: Purified

Immunogen: IL-2-dependent mouse T cell line CTL-L.

Type of Immunogen: Cells

Clone: D7

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:5 - 1:10), IP, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly6A/E / Sca-1 (Ly6a Products)

Synonyms: Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation