Goat anti-Rat IgA Antibody (HRP)
-
- Target See all IgA products
- IgA
-
Reactivity
- Rat
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- HRP
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Anti-Peroxidase, anti-Goat Serum, Rat IgA and Rat Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes
- Purification
- Immunoaffinity purified
- Immunogen
-
Rat IgA alpha chain.
Type of Immunogen: Purified protein
-
-
- Application Notes
-
Approved: ELISA (1:1000 - 1:5000), IHC, WB
Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Rat IgA in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
-
Target Species of Antibody: Rat
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Thimerosal.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
-
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. - Storage
- 4 °C,-20 °C
- Storage Comment
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
-
- Target
- IgA
- Abstract
- IgA Products
- Target Type
- Antibody
-